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简单介绍:
美国哈佛仪器旗下btx公司自1983年成立以来,以电穿孔仪、电融合仪为主要产 品,是苐一家生产高质量电穿孔仪器的生产厂家,开创了许多在电穿孔、电融合、转染、转化等方面的蕞新产品和技术,不仅为全球在此领域的研究者提供了BTX专业产品,而且拥有多至25种的配件,包括:电穿孔、电融合、活体导入配件等 等,成为全球唯壹专业的转基因、电融合、活体转基因仪及配件的生产厂家。 ECM830是为体外和体内电穿孔设计的方形波电穿孔系统,方波技术为研究者提供了更高的细胞转染率和存活率。ECM830可与BTX的各种专业电极及配件结合 使用,应用范围十分广泛,如将基因、**和蛋白质导入活体体内/体外,核移植、胚胎操作、植物原生质体以及**和酵母等等。
详情介绍:

ECM830是为体外和体内电穿孔设计的方形波电穿孔系统,方波技术为研究者提供了更高的细胞转染率和存活率。ECM830可与BTX的各种专业电极及配件结合 使用,应用范围十分广泛,如将基因、**和蛋白质导入活体体内/体外,核移植、胚胎操作、植物原生质体以及**和酵母等等。

 

产品特性

可靠性——温和的方波提高了电穿孔后的细胞存活率,从而保证了转化或转染效率

可操作性——宽的电压和脉冲持续时间范围,脉冲参数可**设定,满足各种应用目的和实验方案的参数设定。

监控显示——液晶屏幕的高分辨率显示电压峰值和脉冲时间,脉冲数,脉冲间隔,使参数的优化、故障的排除和记录成为可能。

**性能——电弧淬灭功能,使由电弧引起的损害降至蕞低;短路保护,避免脉冲发生器遇到短路时被损坏。

兼容性——btx各种电穿孔或电融合电极;btx mos 多孔板电穿孔系统;vip 3000监测系统;脚控开关。

 

应用

哺乳动物细胞的转染

活体/离体基因或**导入

动物细胞融合

植物原生质体融合

核移植

植物组织和原生质体的转化

**、酵母转化

 

技术参数

技术参数

操作界面

7寸彩色触摸屏

输入电压

100 到240 VAC

充电时间

LV <7 s, HV <4 s

电弧控制

Yes

电压范围

LV

5 到500 V , 1 V 分辨率

HV

505 到 3000 V , 5 V 分辨率

脉冲时间

LV

10 到 999 μs ,1 μs 分辨率

LV

1 到 999 ms ,1 ms 分辨率

HV

10 到600 μs, 1 μs 分辨率

多重脉冲

1 99 /每个样本或者1 120

(10 个脉冲/每个样本, 高通量电转)

脉冲间隔

100 ms 到 10 s

可编程性

可储存超过 1,000 个程序

**性能

实验前电阻审核,过电流脉冲中止,电弧保护

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