产品详情
所在位置: 首页> 产品目录> 细胞>
  • 产品名称:Agile Pulse In Vivo活体疫苗电穿孔仪

  • 产品型号:Agile Pulse In Vivo
  • 产品厂商:BTX
  • 产品价格:0
  • 折扣价格:0
  • 产品文档: BTX目录-2 BTX目录-1
你添加了1件商品 查看购物车
简单介绍:
美国哈佛仪器旗下BTX公司自1983年成立以来,以电穿孔仪、电融合仪为主要产品,是苐一家生产高质量电穿孔仪器的生产厂家,开创了许多在电穿孔、电融合、转染、转化等方面的蕞新产品和技术,不仅为全球在此领域的研究者提供了BTX专业产品,而 且拥有多至25种的配件,包括:电穿孔、电融合、活体导入配件等等,成为全球唯壹专业的转基因、电融合、活体转基因仪及配件的生产厂家。
详情介绍:
Agile Pulse In Vivo系统是疫苗和**接种研究者的理想选择。系统根据皮下接种(ID)和肌肉接种(IM)的应用特点分别配置了特别设计的针形矩阵电极,并且对电转染仪的程序进行了特别优化,带给您蕞大的转染效率。电穿孔技术配合质粒注射技术使用,在基因表达及持续性和产生**原性上显示出高达100倍的增长。Pulse Agile技术,首先利用短时间高强度的脉冲使细胞膜产生通透性,再利用低强度脉冲促进DNA导入细胞,配合多针矩阵电极的使用,大大促进了抗原在细胞内的表达。Agile Pulse系统配有丰富多样的多针矩阵电极选择,并且内置电阻测量技术,大大促进了皮下和肌肉**接种技术。

Agile PulseIn Vivo系统能有效增强**反应并缩短**接种程序,从而极大提高您的实验效率。

 

Agile Pulse In Vivo系统广泛应用于:

皮下DNA疫苗接种

肌肉DNA疫苗接种

肌肉DNA疫苗接种

**导入

 

特点:

方波

多重方波

电阻/电击脉冲监控

实验日志存储

无限量自定义程序存贮

远程控制

 

电穿孔应用:

真核细胞

活体(活体电极)

皮下接种(针形矩阵电极)

肌肉接种(针形矩阵电极)

 

技术参数:

用户界面:触摸屏

电压:50 - 1000 v

脉冲时间:5 μs - 10 ms

脉冲间隔时间:20 μs - 1 s

数据输出:USB

产品尺寸(长x x高):12.6 x 7.9 x 15.7 in

重量:25 lbs

工作温度:10° - 40° C

输入电压:100 - 250 VAC

 


已发表文献:

Bongard N, Lapuente D, Windmann S, Dittmer U, Tenbusch M, Bayer, W. Interference of Retroviral Envelope with Vaccine-Induced CD8+ T Cell Responses is Relieved by Co-Administration of Cytokine-Encoding VectorsRetrovirology. 2017;14: 28.

 

Eyquem J, et al. Targeting a CAR to the TRAC Locus with CRISPR/Cas9 Enhances Tumour Rejection. Nature. 2017;543: 113-117.

 

Juillerat A, et al. An Oxygen Sensitive Self-Decision Making Engineered CAR T-CellSci Rep. 2017 Jan 20;7: 39833.

 

Denies S, et al. Evaluation of a Xenogeneic Vascular Endothelial Growth Factor-2 vaccine in Two Preclinical Metastatic Tumor Models in MiceCancer Immunol Immunother. 2017 Dec;66(12): 1545-1555.

 

Juillerat A, et al. Design of Chimeric Antigen Receptors with Integrated Controllable Transient FunctionsSci Rep. 2016 Jan 11;6: 18950.

 

Peterson CW, et al. Long-Term Multi-Lineage Engraftment of Genome-Edited Hematopoietic Stem Cells after Autologous Transplantation in Nonhuman PrimatesBlood. 2016 May 19;127(20): 2416-2426.

 

Hawksworth DJ, Ziemann R, Manoj S. Enhanced Immune Response Following DNA Immunization Through In Vivo ElectroporationMonoclon Antib IImmunodiagn Immunother. 2016 Jul 27;35(5): 239-244.

 

Juillerat A, et al. Design of chimeric antigen receptors with integrated controllable transient functionsSci Rep. 2016 Jan 11;6: 18950.

 

Christopher W, et al, Long-term multilineage engraftment of autologous genome-edited hematopoietic stem cells in nonhuman primatesBlood. 2016;127: 2416-2426.

 

Wang L, et al. Evaluation of Candidate Vaccine Approaches for MERS-CoVNat Comm. 2015 Jul28;6:7 712.

 

Davtyan H, et al. BTX AgilePulse(TM) System is an Effective Electroporation Device for Intramuscular and Intradermal Delivery of DNA Vaccine. Curr Gene Ther. 2014;14(3): 190-199.

 

Daftarian MP, Vosoughi A, Lemmon V. Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies. Methods Mol Biol. 2014;1121: 337-346.

 

Johansson DX, Ljungberg K, Kakoulidou M, Liljestrom P. Intradermal Electroporation of Naked Replicon RNA Elicits Strong Immune ResponsesPLoS One, 2012 Jan 4;7(1): e29732.

 

Lladser A, Ljungberg K, Tufvesson H, Tazzari M, Roos A, Quest FG, Kiessling R. Intradermal electroporation with a survivin DNA vaccine induces CTLs against a self-epitope, suppresses angiogenesis and confers long-term protection against mouse melanomaCancer Immunol Immunother. 2010 Jan;59(1): 81-92.

 

Lundberg K, Roos A-K, Pavlenko M, Wehrum D, Pisa P. A modified epitope identified for generation and monitoring of PSA-specific T cells in patients on early phases of PSA-based immunotherapeutic protocolsVaccine. 2009 Mar 4;27(10): 1557-65.

 

Bråve A, Hallengärd D, Gudmundsdotter L, Stout R, Walters R, Wahren B, Hallermalm K. Late administration of plasmid DNA by intradermal electroporation efficiently boosts DNA-primed T and B cell responses to carcinoembryonic antigenVaccine. 2009 Jun 8;27(28): 3692-6.

 

Roos, A-K, Eriksson, F, Walters, D, Pisa, P, King, A. Optimization of skin electroporation in mice to increase tolerability of DNA vaccine delivery to patientsMolecular Therapy. 2009 Sep;17(9): 1637-42.



沪公网安备 31010502003083号