ECM® 2001是一台多功能的细胞电融合和电穿孔仪器，独特的交流正弦波使细胞在双向电泳作用下排列到一起，然后在微秒级时间内转换成直流方波使其融合；融合后又一个短暂的交流脉冲稳定杂合细胞的融合状态，大大提高了细胞融合的效率。
ECM® 2001单独发生直流方波脉冲，可作为电穿孔系统，选配多种BTX专业电极，适用于各种要求的电穿孔实验，广泛用于离体和活体电穿孔。
多功能
兼有细胞电融合和电穿孔功能，满足从简单转染到细胞融合一系列实验操作。
灵活性
拥有较宽的电压和脉冲时间范围，可根据实验方案调整所有脉冲参数，可在20Ω低阻抗下操作；融合过程在显微镜下可视操作，提高细胞融合精度。
快速高效
通过交流正弦波和直流方波电脉冲的交替作用，可以简单而快速地完成细胞排列、融合、融合后处理全过程，仅需几秒钟。
**性能
电弧淬灭功能，降低电弧引起的损害；短路保护，避免脉冲发生器遇到短路时被损坏。
兼容性
可选配不同型号微型载玻片电极， 同时也可配2ml和9ml电融合池，提高效率。Enhancer3000监测系统，脚控开关
监控仪选配
Enhancer 3000可以允许用户监控和记录主要的电流参数，利用选配的通讯模块，可以把数据下载到电脑上或在打印机上打印出来。
应用
动物细胞融合
核转移
胚胎操作
杂交瘤生成
植物原生质体融合
活体/离体/卵内基因或Drug导入
动物细胞或组织的转染
干细胞生成
部分原核生物（bacteria）、酵母的转化
|
技术参数 |
|||
|
交流参数 |
独特的非正弦波形 |
||
|
频率 |
1MHz |
||
|
电压 |
0-75V |
||
|
脉冲时间 |
0-99 sec |
||
|
融合后电压 |
融合前的1/10 |
||
|
融合后时间 |
1-9 sec |
||
|
交流 / 直流 间隔时间 50μsec |
|||
|
直流参数 |
方波 |
||
|
高压模式 |
电压 |
10-3000V，10V调进 |
|
|
波长 |
1-99μsec，1μsec分辨率 |
||
|
低压模式 |
电压 |
10-500V，10V调进 |
|
|
波长 |
1-99msec，1msec分辨率 10-990μsec，10μsec分辨率 |
||
|
脉冲数 |
1-9个 |
||
|
脉冲间隔 |
1 sec |
||
|
循环数0－9个 |
|||
发表文献：
Natalie J. Thornburg, Heng Zhang, Sandhya Bangaru, Gopal Sapparapu, Nurgun Kose, Rebecca M. Lampley, Robin G. Bombardi, Yingchun Yu, Stephen Graham, Andre Branchizio, Sandra M. Yoder, Michael T. Rock, C. Buddy Creech, Kathryn M. Edwards, David Lee, Sheng Li, Ian A. Wilson, Adolfo García-Sastre, Randy A. Albrecht, and James E. Crowe Jr. H7N9 influenza virus neutralizing antibodies that possess few somatic mutations. The Journal of Clinical Investigation, April 2016.
Clow A., Gaynor P., Oback, B. A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusion. Biomed Microdevices. 12, 777-786 (2010) .
Green, A.M., Wells, D.N., Oback, B. Cattle cloned from increasingly differentiated muscle cells. Biol Reprod. 77, 395-406 (2007).
Young Chung, Sandy Becker., Embryonic Stem Cells Using Nuclear Transfer. Methods in Enzymology., Volume 418, 2006.
Kenichiro, I. et al. Induction of cytotoxic T lymphocytes against human cancer cell lines using dendritic cell-tumor cell hybrids generated by a newly developed electrofusion technique. INTERNATIONAL JOURNAL OF ONCOLOGY 29: 531-539, 2006.
Nakamura, Y. et al. Phospholipase C-σ1 and σ3 Are Essential in the Trophoblast for Placental Development. Molecular And Cellular Biology,(2005).
Soares, M.L. et al. Functional Studies for Signaling Pathways in Peri-implantation development of the Mouse Embryo by RNAi. BMC Developmental Biology,(2005).
Bedford, S. J. et al. Patterns of Intracellular Calcium Oscillation in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection: Possible Explanations for the Low Success of this Assisted Reproduction Technique in the Horse. Biology of Reproduction, (2004).
Yul, Z. et al. Specific Antitumor Effects for Tumor Vaccine Produced by Electrofusion between Osteosarcoma Cell and Dendritic Cell in Rat. Cellular & Molecular Immunology, (2004).
Grabarek, J.B. et al. Efficient Delivery of dsRNA into Zona-Enclosed Mouse Oocytes and Preimplantation Embryos by Electroporation. Genesis, (2002).
Liu, H. et al. Reconstruction of Mouse Oocytes by Germinal Vesicle Transfer: Maturity of Host Oocyte Cytoplasm Determines Meiosis. Human Reproduction,(1999).
