ECM830是为体外和体内电穿孔设计的方形波电穿孔系统，方波技术为研究者提供了更高的细胞转染率和存活率。ECM830可与BTX的各种专业电极及配件结合 使用，应用范围十分广泛，如将基因、药WU和蛋白质导入活体体内/体外，核移植、胚胎操作、植物原生质体以及原核细胞（bacteria）和酵母等等。
产品特性
可靠性——温和的方波提高了电穿孔后的细胞存活率，从而保证了转化或转染效率
可操作性——宽的电压和脉冲持续时间范围，脉冲参数可准确设定，满足各种应用目的和实验方案的参数设定。
监控显示——液晶屏幕的高分辨率显示电压峰值和脉冲时间，脉冲数，脉冲间隔，使参数的优化、故障的排除和记录成为可能。
安全性能——电弧淬灭功能，使由电弧引起的损害降至很低；短路保护，避免脉冲发生器遇到短路时被损坏。
兼容性——btx各种电穿孔或电融合电极；btx 高通量电穿孔系统；vip 3000监测系统；脚控开关。
应用
哺乳动物细胞的转染
活体/离体基因或药WU导入
动物细胞融合
植物原生质体融合
核移植
植物组织和原生质体的转化
原核细胞（bacteria）、酵母转化
技术参数
|
技术参数 |
|
|
操作界面 |
7寸彩色触摸屏 |
|
输入电压 |
100 到240 VAC |
|
充电时间 |
LV <7 s, HV <4 s |
|
电弧控制 |
Yes |
|
电压范围 |
|
|
LV |
5 到500 V ， 1 V 分辨率 |
|
HV |
505 到 3000 V ， 5 V 分辨率 |
|
脉冲时间 |
|
|
LV |
10 到 999 μs ，1 μs 分辨率 |
|
LV |
1 到 999 ms ，1 ms 分辨率 |
|
HV |
10 到600 μs， 1 μs 分辨率 |
|
多重脉冲 |
1 到99 /每个样本或者1 到 120 (10 个脉冲/每个样本, 高通量电转) |
|
脉冲间隔 |
100 ms 到 10 s |
|
可编程性 |
可储存超过 1,000 个程序 |
|
安全性能 |
实验前电阻审核，过电流脉冲中止，电弧保护 |
已发表文献：
Denies S, et al. In Vitro Exploration of a Myeloid-Derived Suppressor Cell Line as Vehicle for Cancer Gene Therapy. Cancer Gene Ther. 2017 Apr;24: 149-155.
Huang J, et al. Generation and Comparison of CRISPR-Cas9 and Cre-Mediated Genetically Engineered Mouse Models of Sarcoma. Nat Commun. 2017 Jul;8: 15999.
Sun Y, et al. Coinjection of IL2 DNA Enhances E7-Specific Antitumor Immunity Elicited by Intravaginal Therapeutic HPV DNA Vaccination with Electroporation. Gene Ther. 2017 Jul;24(7): 408-415.
Xia W, Jiao J. Histone Variant H3.3 Orchestrates Neural Stem Cell Differentiation in the Developing Brain. Cell Death Differ. 2017 Sep;24(9): 1548-1563.
Hsieh LS, et al. Convulsive Seizures from Experimental Focal Cortical Dysplasia Occur Independently of Cell Misplacement. Nature Commun. 2016 Jun;7: 11753.
Liu X, et al. Improved Anti-Leukemia Activities of Adoptively Transferred T cells Expressing Bispecific T-cell Engager in Mice. Blood Cancer J. 2016 Jun;6(6): e430.
Martell JD, et al. A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses. Nat Biotechnol. 2016 May;34: 774-780.
Patel MS, et al. Growth differen
tiation factor-15 is associated with muscle mass in chronic obstructive pulmonary disease and promotes muscle wasting in vivo. J Cachexia, Sarcopenia and Muscle. 2016 Sep; 7(4): 436-448.
Ploier B, et al. Dimerization Deficiency of Enigmatic Retinitis Pigmentosa-Linked Rhodopsin Mutants. Nature Commun. 2016 Oct;7: 12832.
Toda T, Shinmyo Y, Dinh Duong TA, Masuda K, Kawasaki H. An Essential Role of SVZ Progenitors in Cortical Folding in Gyrencephalic Mammals. Sci Rep. 2016;6: 29578.
Shinmyo Y, et al. CRISPR/Cas9-mediated Gene Knockout in the Mouse Brain using in Utero Electroporation. Sci Rep. 2016;6: 206111.
Varela JA, et al. Targeting Neurotransmitter Receptors with Nanoparticles In Vivo Allows Single-Molecule Tracking in Acute Brain Slices. Nature Commun. 2016;7: 10947.
Liu X, et al. T Cell Receptor-Induced NF-kB Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-Mediated Calcium Entry. J Biol Chem. 2016 Apr;291(16): 8440-8452.
Baumgart J, Grebe N. C57BL/6-specific conditions for efficient in utero electroporation of the central nervous system. J Neurosci Methods. 2015 Jan;240: 116-124.
Kim NY, et al. Enhanced Immune Response to DNA Vaccine Encoding Bacillus Anthracis PA-D4 Protects Mice Against Anthrax Spore Challenge. PLoS One, 2015 Oct;10(10): e0139671.
Jiang JJ, et al. MicroRNA-26a Supports Mammalian Axon Regeneration In Vivo by Suppressing GSK3β Expression. Cell Death & Disease. 2015 Aug;6: e1865.
Rodriguez-Gil DJ, et al. Odorant Receptors Regulate the Final Glomerular Coalescence of Olfactory Sensory Neuron Axons. Proc Natl Acad Sci. 2015 May;112(18):5821-5826.
Simpson MT, et al. The Tumor Suppressor HHEX Inhibits Axon Growth When Prematurely Expressed in Developing Central Nervous System Neurons. Mol Cell Neurosci. 2015 Sep;68: 272-283.
Wang J, et al. Homology-Driven Genome Editing in Hematopoietic Stem and Progenitor Cells using ZFN mRNA and AAV6 Donors. Nature Biotechnol. 2015 Dec;33(12): 1256-1263.
Zhang H, et al. Time Point‐Based Integrative Analyses of Deep‐Transcriptome Identify Four Signal Pathways in Blastemal Regeneration of Zebrafish Lower Jaw. Stem Cells. 2015 Mar;33(3): 806-81.
Feng L, Kwon O, Lee B, Oh WC, Kim J. Using Mammalian GFP Reconstitution across Synaptic Partners (mGRASP) to Map Synaptic Connectivity in the Mouse Brain. Nat Protoc. 2014;9: 2425-2437.
Shi, W. et al., Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo. Acta Biochim Biophys Sin; 2010; 42(2): 116-221.
Nasser, N. et al. Alternatively spliced Spalax heparanase inhibits extracellular matrix degradation, tumor growth, and metastasis. PNAS. 2009;106(7): 2253-2258.
Clape, C. et al. miR-143 Interferes with ERK5 Signaling, and Abrogates Cancer Progression in Mice. PLoS One. 2009 Oct;4(10): e7542.
